马铃薯内源转基因质粒载体构建

Recently scientists propose a new strategy for?transgene named intragenesis. It is the genetic modification of a recipient plant with a natural gene from a crossable-sexually compatible-plant. This could avoid the risk of transgenes. To construc a potato intragenic plasmid, PCR amplification, restriction enzyme digestion, ligation and heat shock transformation were used to clone the potato intragenic plasmid by using a potato derived T-DNA like element 1RB to replace right border sequence of T-DNA in binary plasmid vector pBI121. It will lay an important foundation for establishment of potato endogenous expression system.