华东地区戊型肝炎病毒基因IV型亚型多样性分析
Identification of the subtype diversity of genotype 4 hepatitis E virus in eastern China
戊型肝炎病毒（Hepatitis E virus， HEV）作为一种人畜共患病原,能感染多种动物。猪可能是人感染HEV的最重要的天然贮存宿主。基因4型HEV是引发中国人群戊型肝炎的最主要基因型。已有研究发现，中国部分地区地区基因4型HEV可以在人和猪群中跨种间传播。本研究利用RT-PCR方法对125份HEV IgM抗体阳性的人血清样品和290份猪粪便样品进行HEV RNA的检测。结果显示24份人血清样品和29份猪粪便样品呈现HEV阳性。基于部分衣壳蛋白基因序列的系统进化分析表明：本研究的51株戊型肝炎病毒毒株均为基因4型，分别聚于6个不同的亚型。其中有些亚型中同时存在来自人群和猪群的毒株，表明可能存在跨种间传播的发生。该研究结果表明我国华东地区基因4型HEV的具有亚型多样性特点。
Hepatitis E viruses (HEVs), a zoonotic pathogen, use several species of animal as reservoirs. Swine is considered as the major reservoir for HEV infection in humans. Genotype 4 HEV is the dominant cause of hepatitis E disease in humans in China. Several recent studies showed that genotype 4 HEV is transmitted between humans and swine in China. In the present study, a total of 125 anti-HEV IgM positive human serum and 290 swine fecal samples were subjected to RT-PCR screening of HEV RNA. Results indicated 24 human serum samples were positive, while 9.66% (28/290) of the 290 swine fecal samples were positive for HEV RNA. Phylogenetic analysis based on partial capsid gene showed that the 51 HEV strains in the current study all belonged to genotype 4, clustering into 6 different subtypes. Some of HEV isolates prevalent in the human and swine populations were classified into the same clusters, suggesting that cross-species transmission occurred. Our study revealed the subgenotype diversity of genotype 4 HEV in eastern China.
黄媛、李旺、张文、周成林、周改、孙嘉遥、傅行礼、田华、杨世兴、沈权
医药卫生理论医学研究方法基础医学
戊型肝炎病毒亚型进化分析
Hepatitis E virusSubtypePhylogenetic analysis
黄媛,李旺,张文,周成林,周改,孙嘉遥,傅行礼,田华,杨世兴,沈权.华东地区戊型肝炎病毒基因IV型亚型多样性分析[EB/OL].(2014-11-17)[2025-08-11].http://www.paper.edu.cn/releasepaper/content/201411-310.点此复制
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