三个玫瑰品种花色物质的代谢组学分析

Rosa rugosa is a deciduous shrub belonging to Rosa L. in the Rosaceae family. It hashigh ornamental value and commercial value, but its single color limits the development andutilization of rose and its application in landscape architecture. In order to explore the coloringsubstances of three different varieties of roses, Rosa rugosa Rosa sertata , Rosa CrimsonGlory and Rosa alba, this study used ultra-high performance liquid chromatography-four-poletime-of-flight mass spectrometry (UPLC-Q-TOF-MS) to detect thetypes and contents offlavonoids in petals. The KEGG database was used to enrich the differential metabolites, screenout the key metabolites, and analyze thecorrelation with the phenotypic value of flower color. Theresults were as follows: (1) A total of 58 metabolites were detected in petals of different rose varieties, of which only one anthocyanin was cyanidin-3-O-glucoside, accounting for 30.45%. (2)K-means clustering analysis showed that a total of 12 key metabolites were annotated to theKEGGmetabolic pathway. Among them, pinocembrin and myricetin were the main substances thatdetermined the red color of Rosa rugosa Rosa sertata and Rosa Crimson Glory, anderiodictyol, luteolin and kaempferol were the main substances that determined the white color ofRosa alba. In conclusion, this study can provide a theoretical basis for the breeding of roses withspecific colors and promote the application of roses in landscaping.