高效液相色谱法分析海参三萜皂苷中的单糖组成

o fast and effectively analyze the sugar chain of triterpene glycosides from sea cucumber, a method was developed using a reverse-phase high-performance liquid chromatography (HPLC) with 1-phenyl-3-methyl-5-pyrazolone (PMP) derivatization. An optimized chromatographic condition was established in the present study. The calibration curves of monosaccharides (D-glucose, D-3-O-methylgucose, D-xylose and D-quinovose) were linear within the range of 0.05 - 1 mmol/L (R2 > 0.996). The detection limits of these four monosaccharides were below 5 pmol. Monosaccharides of holothurin A and holothurin B were analyzed by this method. The result showed it was simple, rapid, stable, and less sample consumed than previous methods.