荞麦水溶性多糖的分离纯化及其相对分子量的测定
Isolation and Purification of a water-soluble Polysaccharide from Fagopyum esculentum and Determination of its molecular weight
本文通过研究了通过热水浸提法从荞麦中提取了一种水溶性多糖,并用离子交换柱和凝胶过滤柱对荞麦多糖进行分离纯化、测定了其相对分子量。实验结果表明,荞麦多糖粗品用Savag 法脱蛋白后，采用DEAE－Sepharose Fast Flow 和Sephacryl S-400 HR 柱层析纯化，得到荞麦多糖FEP，经过凝胶柱色谱分析表明，FEP 为均一多糖。用苯酚硫酸法测定多糖的含量，用AKTA快速液相蛋白层析仪的紫外检测器在280nm监测蛋白质含量。最后根据用不同分子量的标准葡聚糖的做出的标准曲线，测定出FEP 分子量(Mw)为1720442Da。
In this paper, a water-soluble crude polysaccharide, obtained from Fagopyum esculentum by boiling-water extraction and ethanol precipitation, was fractionated by DEAE–Sepharose Fast Flow column chromatography, and purified by Sephacryl S-400 HR column chromatography. It was showed only one symmetrical peak from gel-filtration chromatography, indicating that FEP was a homogeneous polysaccharide. The total sugar content of each tube was determined by the phenol-sulfuric acid assay and the protein content was detected by the UV detector at the wavelength of 280 nm. The weight-average molecular weight (Mw) of the purified polysaccharide (FEP) was calculated to be 1720442 Da, according to the calibration curve with standard dextrans.
王颖、张方方、黄昆仑、许文涛、罗云波
生物科学研究方法、生物科学研究技术植物学生物化学
荞麦多糖分离纯化分子量测定
Fagopyum esculentumPolysaccharidePurificationDetermination of molecular weights
王颖,张方方,黄昆仑,许文涛,罗云波.荞麦水溶性多糖的分离纯化及其相对分子量的测定[EB/OL].(2008-04-10)[2025-08-11].http://www.paper.edu.cn/releasepaper/content/200804-333.点此复制
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