水稻根系响应铜胁迫的蛋白质组学研究

opper (Cu) is an essential micronutrient required for growth and development of plants. However, excess Cu is toxic to plants. To understand the mechanisms involved in copper stress response, a proteomic approach was used to investigate difference in Cu stress-induced protein expression of rice by two-dimensional PAGE and MALDI-TOF mass spectrometry. The results showed that thirty-four protein spots were found to be differently expressed in control (0.32 μmoloL-1 CuSO4) and Cu-treated (8 μmoloL-1 CuSO4) rice seedling. These identified proteins included those involved in antioxidative defense, redox regulation, stress response, sulfur and GSH metabolism, carbohydrate metabolism, signal transduction, and some other proteins with various functions. The majority of these proteins are involved in redox regulation and sulfur and GSH metabolism, suggesting that these proteins may work together with antioxidant enzymes to establish a new homeostasis in response to Cu stress.