急性温度胁迫对妊娠期许氏平鲉血清皮质醇和血液生理指标的影响

o explore theadaptability and restoring ability in water with mutant temperature,gestated Sebastesschlegeliiwere moved from normal temperature (16℃) to high (25℃) or low (7℃) temperature water, being exposed for 2h, and subsequently transferred to original seawater of 16 ℃.Sampleswere collected at 0, 6, 12, 24, 48 and 72hduring recovery to detect changes of serum cortisol and hematological and physiological parameters of gestated Sebastesschlegelii.the data showed that acute thermal stress lead to parturition behavior of two fish; It was noted that serum cortisol(COR), glucose(GLU), globulin(GLB), glutamic-pyruvic transaminase(GPT), glutamic-oxaloacetictransaminase(GOT) and white blood cell (WBC)levels in serum increased significantly(P<0.05) whereas concentrations of total protein (TP), albumin(ALB), total cholesterol(TC)andtriglyceride(TG)became lower obviouslyduring recovery process after acute stressor of 25℃ and 7 ℃(P<0.05). Besides, there was no difference in Ca2+、Mg2+ and P contents. Interestingly, red blood cell (RBC) and hemoglobin (HGB) levels arose remarkably after acute heat stress (P<0.05) while those were detected down-regulated by acute chilling stress (P<0.05). It was observed that thermal stress had a sharper effect on physiological status compared with acute chilling stress. Moreover, most indexes, except for COR and inorganic ions, did not restore to normal level after 72 hours. Our results suggest that acute temperature stress results indistinct impact on physiological function and metabolic levels of gestated Sebastesschlegelii, and even parturition behavior in advance. It is of high importance to monitor closelywater temperature fluctuation especially elevation, as for female fish entering the reproductive phase, in order to decreaseinjury induced by temperature stress during pregnancy.