心肌肥厚心肌细胞中RhoA/ROCK通路和ERK1/2通路的相互作用

Objective：To investigate whether the interaction between RhoA/ROCK pathway and ERK pathway in the cultured hypertrophy cardiomyocyts．Methods：the hearts of neonatal rats were isolated and cultured in vitro，then the cardiomyocyts were divided into four groups which stimulated by same dosage isoprenaline respectively：1)control group，2)RhoA group，3)ERK blocking drug group，4)RhoA +ERK blocking drug group．The expression of ROCK，ERK1/2 proteins，the content of phosphorylated ERK1/2 (P-ERK1/2) and ROCK proteins (P-ROCK)were detected by Western blotting，and the expression of ROCK mRNA was detected by reverse transcription-polymerase chmn reaction(RT-PCR)．Results：1)Compared with the control group，the content of P-ROCK and P-ERK1/2 proteins in RhoA group was increased(P<0.05)，that in ERK blocking drug group was increased (P<0.05)．There was no diference between control group and RhoA +ERK blocking drug group．Compared with RhoA group，the contents of P-ROCK and P-ERK1/2 proteins in ERK blocking agent group and RhoA +ERK blocking drug group were decreased(P<0.05)．There was no difference in the expression of ROCK and ERK1/2 proteins among diferent groups．2)There were obvious differences in expression of ROCK mRNA among diferent groups．Conclusion：1) RhoA may direct activate ROCK proteins and ERK 1/ 2 pathway, The ERK1/ 2 pathway may be located in the downstream of the RhoA/ROCK pathway. 2) The activation of ROCK proteins partial depends on ERK pathway stimulation．