Direct Energy Transfer from Allophycocyanin-Free Rod-Type CpcL-Phycobilisome to Photosystem I
Direct Energy Transfer from Allophycocyanin-Free Rod-Type CpcL-Phycobilisome to Photosystem I
ABSTRACT Phycobilisomes (PBSs) are photosynthetic antenna megacomplexes comprised of pigment-binding proteins (cores and rods) joined with linker proteins. A rod-type PBS that does not have a core is connected to photosystem I (PSI) by a pigment-free CpcL linker protein, which induces a red-shift of the absorption band of phycocyanobilin (PCB) in the rod (red-PCB). Herein, the isolated supercomplex of the rod-type PBS and the PSI tetramer from Anabaena sp. PCC 7120 were probed by picosecond laser spectroscopy at 77 K and by decay-associated spectral analysis to show that red-PCB mediates the fast (time constant = 90 ps) and efficient (efficiency = 95%) transfer of excitation energy from PCB in rod to chlorophyll a (Chl a) in PSI. According to the F?rster energy transfer mechanism, this high efficiency corresponds to a 4-nm distance between red-PCB and Chl a, suggesting that β-84 PCB in rod acts as red-PCB. TOC GRAPHICbiorxiv;2021.05.31.446472v1/UFIG1F1ufig1
Watanabe Mai、Noji Tomoyasu、Itoh Shigeru、Dewa Takehisa、Ikeuchib Masahiko
Department of Life Sciences (Biology), Graduate School of Arts and Sciences, University of Tokyo||Core Research for Evolutional Science and Technology, Japan Science and Technology Agency||JSPS Overseas Research FellowDepartment of Life Science and Applied Chemistry, Graduate School of Engineering, Nagoya Institute of TechnologyDepartment of Physics, Graduate School of Nagoya UniversityDepartment of Life Science and Applied Chemistry, Graduate School of Engineering, Nagoya Institute of TechnologyCore Research for Evolutional Science and Technology, Japan Science and Technology Agency
生物化学生物物理学分子生物学
chlorophyllenergy transferfluorescence lifetimephotosystem Iphycocyaninrod-type phycobilisome
Watanabe Mai,Noji Tomoyasu,Itoh Shigeru,Dewa Takehisa,Ikeuchib Masahiko.Direct Energy Transfer from Allophycocyanin-Free Rod-Type CpcL-Phycobilisome to Photosystem I[EB/OL].(2025-03-28)[2025-04-26].https://www.biorxiv.org/content/10.1101/2021.05.31.446472.点此复制
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