氯化钆通过激活Akt通路促进Huh7细胞中FoxO1的磷酸化

Objective: Akt/FoxO1 pathway is one of the most important signal pathways in liver cells. Gadoliniu chloride is a rare earth compound. It has been reported that gadolinium chloride could activate the Akt pathway in NIH3T3 cells. In this paper, the effect of gadolinium chloride on the Akt pathway and phosphorylation of FoxO1 in HuH7 cells was investigated. Methods: Lysis was extracted from the HuH7 cells after incubation with gadolinium chloride, and detected the levels of Akt, phosphorylated Akt, FoxO1 and phosphorylated FoxO1 with western blotting analysis. Then the effect of gadolinium chloride on phosphorylation of FoxO1 was detected after Akt pathway was inhibited by LY294002. Results: The level of total Akt did not change, but the phosphorylation levels of Akt increased greatly under the action of gadolinium chloride, and the same change was observed on phosphorylated FoxO1. And the role of gadolinium chloride decreased greatly after inhibition of Akt pathway. Conclusion: In HuH7 cells, gadolinium chloride could promote the phosphorylation of FoxO1 by activating the Akt pathway.