半乳糖基光控荧光探针的合成及其性能研究
Synthesis and Properties of Galactosyl - based Fluorescence Probe
基于螺吡喃-萘酰亚胺骨架,通过经典的点击反应以及糖基化反应,我们设计合成了半乳糖-萘酰亚胺-螺吡喃(SP-Gal, SP-Gal 2, SP-PEG)类,用于细胞内亚硫酸根离子检测光控荧光探针。当探针分子中的螺吡喃基团处于开环状态(MR)时,探针中的萘酰亚胺基团会与其之间存在较强的荧光共振能量转移(FRET)效应,大大减弱了探针本身的荧光。亚硫酸根离子存在时,会与开环状态的化合物发生迈克尔加成反应,阻断了FRET效应,化合物荧光得到恢复,从而达到检测亚硫酸根离子的目的,该研究为基于光调控的新一代生物体成像工具的开发奠定了基础。
Based on the spiropyran-naphthalimide skeleton, we designed and synthesized galactose-naphthalimide-spiropyran (SP-Gal, SP-Gal 2, SP-PEG) photochromic fluorescent probes by the click reaction and glycosylation reaction. In addition, photoisomerization of SP produces the MR isomer with an extended conjugated system, which can serve as a FRET acceptor to tune the fluorescence of closely coupled fluorophores. The selectively endocytosed probe can thus execute light orders intracellularly to reversibly switch between two photoisomeric states, outputting a dynamic, "blinking" fluorescence signal.
张隽佶、付幼心
生物化学生物物理学分子生物学
糖类荧光探针亚硫酸根萘酰亚胺光致变色
Glycosyl fluorescent probesSulfiteNaphthalimidePhotochromism
张隽佶,付幼心.半乳糖基光控荧光探针的合成及其性能研究[EB/OL].(2017-05-12)[2025-08-24].http://www.paper.edu.cn/releasepaper/content/201705-799.点此复制
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