A Herpes Simplex Virus Type-1-derived influenza vaccine induces balanced adaptive immune responses and protects mice from lethal influenza virus challenge
A Herpes Simplex Virus Type-1-derived influenza vaccine induces balanced adaptive immune responses and protects mice from lethal influenza virus challenge
Abstract Influenza virus is a major respiratory viral pathogen responsible for the deaths of hundreds of thousands worldwide each year. Current vaccines provide protection primarily by inducing strain-specific antibody responses with the requirement of a match between vaccine strains and circulating strains. It has been suggested that anti-influenza T-cell responses, in addition to antibody responses may provide the broadest protection against different flu strains. Therefore, to address this urgent need, it is desirable to develop a vaccine candidate with an ability to induce balanced adaptive immunity including cell mediated immune responses. A live viral vector technology should exhibit safety, immunogenicity, effectiveness in the presence of pre-existing immunity, and the ability to induce mucosal immune responses. Here, we used VC2, an established Herpes Simplex Virus type 1 vaccine vector, to express the influenza HA protein. We show that this virus is capable of generating potent and specific anti-influenza humoral and cell-mediated immune responses. We further show that a single vaccination with the VC2-derived influenza vaccine protects mice from lethal challenge with influenza virus. Our data support the continued development of VC2-derived influenza vaccines for protection of human populations from both seasonal and pandemic strains of influenza. Finally, our results support the potential of VC2-derived vaccines as a platform for the rapid development of vaccines against emerging and established pathogens, particularly respiratory pathogens.
Hai Rong、Rider Paul J.F.、Dulin Harrison、Uche Ifeanyi K.、McGee Michael C.、Tan Gene S.、Breitenstein Blake、Kousoulas Konstantin G.、Huang Weishan
Department of Microbiology and Plant Pathology, University of CaliforniaDivision of Biotechnology and Molecular Medicine Department of Pathobiological Sciences, School of Veterinary Medicine, Louisiana State University||Department of Pathobiological Sciences, School of Veterinary Medicine, Louisiana State UniversityDepartment of Microbiology and Plant Pathology, University of CaliforniaDivision of Biotechnology and Molecular Medicine Department of Pathobiological Sciences, School of Veterinary Medicine, Louisiana State University||Department of Pathobiological Sciences, School of Veterinary Medicine, Louisiana State UniversityDepartment of Pathobiological Sciences, School of Veterinary Medicine, Louisiana State UniversityJ. Craig Venter InstituteDepartment of Microbiology and Plant Pathology, University of CaliforniaDivision of Biotechnology and Molecular Medicine Department of Pathobiological Sciences, School of Veterinary Medicine, Louisiana State University||Department of Pathobiological Sciences, School of Veterinary Medicine, Louisiana State UniversityDepartment of Pathobiological Sciences, School of Veterinary Medicine, Louisiana State University||Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University
预防医学生物科学研究方法、生物科学研究技术微生物学
HerpesvirusinfluenzafluVC2viral vectorvaccine
Hai Rong,Rider Paul J.F.,Dulin Harrison,Uche Ifeanyi K.,McGee Michael C.,Tan Gene S.,Breitenstein Blake,Kousoulas Konstantin G.,Huang Weishan.A Herpes Simplex Virus Type-1-derived influenza vaccine induces balanced adaptive immune responses and protects mice from lethal influenza virus challenge[EB/OL].(2025-03-28)[2025-06-18].https://www.biorxiv.org/content/10.1101/2021.08.05.455241.点此复制
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