保安族口腔健康儿童唾液微生物群落结构分析

Objective: To explore the dominant salivary microbiota in healthy children by analyzing microbial community structure of healthy children in Bonan of Gansu province. Methods: A total of 8 Bonan's children with healthy oral cavity were included in this study, Genomic DNA was extracted from saliva samples. 16S rRNA clone library was constructed after the 16s rRNA was amplified, then selected 400 positive clones randomly to sequences and compared the sequencing results by BLAST. After removing the vectors and chimera, the analysis of diversity of bacterial community structure was conducted by software such as Mothur, Clustalx2.0, etc. Phylogenetic tree was constructed used MEGA4.0. Results: After sequencing and screening, 387 qualified sequences were obtained. They were classified into 84 OTUs which belonging to5 phyla, 12 classes, 15 orders, 18 families, 19 genera. The proportion of Firmicutes(88.60%)was the most. Only 6 genera were observed at high abundance (detection rate >1%). These included: Streptococcus (70.28%), GranuIicatella (6.20%), Gemella (3.10%), Veillonella (1.81%), Neisseria (1.03%) and Haemophilus (1.03%). Among them, Streptococcus was detected in all 8 samples. Conclusion: The salivary microbial community structure of healthy children in Bonan is more abundant. Streptococcus is the dominant bacteria in saliva of healthy children in Bonan, and its specific mechanism of maintaining oral health status in children may need a further study.