miR-9过表达对鼻咽癌细胞迁移和侵袭的影响

Objective: To investigate the effect of miR-9 on migration and invasion of nasopharyngeal carcinoma cells. Methods: After lentiviral infection in nasopharyngeal carcinoma C666-1 cell suspension, flow cytometry sorting and identification for obtaining stable overexpression of miR-9 cell lines, C666-1/miR-9 and control cells C666-1/PLVTHM were evaluated to analyze the effects of miR-9 on cell migration and invasion, using wound healing assay and transwell invasion in vitro. Results: After being made a scratch for 12h, the number of C666-1/miR-9 cells passing through the scratch(47.167+2.552) was significantly lower than that of C666-1/PLVTHM cells (95.917 + 3.118) (t=41.912, P=0.000); Meanwhile, after being coated in the transwell champer, the number of C666-1/miR-9 cells penetrating through the Matrigel (32.667 +3.257) was significantly less than that of C666-1/ PLVTHM cells (81.833+4.366) (t=31.270, P=0.000). Conclusion: Overexpression of miR-9 could inhibit the migration and invasion of nasopharyngeal carcinoma cells in vitro, indicating that miR-9 plays an important role in the inhibition of nasopharyngeal carcinoma cells.