KAR信号通路抑制蛋白SMAX1的功能研究

In this study, in order to explore the regulatory role of the Karrikin signaling pathway repress protein SMAX1 in the growth and development of legumes, we used CRISPR/Cas9 system to target MtSMAX1, and obtained a series of transgenic M. truncatula by Agrobacterium transformation. Then, we identified geneStudy on Function of Karrikin Signalling Pathway Repressor Protein SMAX1 edited individuals with dStudy on Function of Karrikin Signalling Pathway Repressor Protein SMAX1ivergent base mutations, some of which are homozygous mutants with defective function. It was found that mtsmax1 mutants\' the internode length of the primary branches has significantly shorten, resulting in a highly decrease in the plant height of the homozygous mutant. Moreover, the initial flowering stage of mtsmax1 mutant was later than that of the wild type R108, and it was also accompanied by a reduction in the expression of flowering positive regulators MtAP1 and MtFULc. The regulation of plant height and flowering phenotype indicate that MtSMAX1 plays a essential developmental roles in the growth and development of legumes.