产β-甘露聚糖酶菌株的筛选及产酶条件的优化

Objective: To select strains for the production of β-Mannanase from compost, hot springs and other high temperature environment and optimize conditions of enzyme production for the industrialization. Methods: Designing enrichment and selection medium in order to select the fungal strains with β-mannanase; Identification those strains by morphological and molecular biology methods; Determining production activity and optimizing conditions of the enzyme production by liquid fermentation. Results: 9 fungal strains with β-mannanase were obtained, including the strain of PSC-D-G18 which had the stronger enzyme activity that was 56 U/ml. The optimum pH and temperature for the β-mannanase activity were 5.0 and 60℃, respectively. This fungal strain was identificated as Sporothrix.schenckii. Conclusion:The strain of PSC-D-G18 which had the stronger enzyme activity of β-mannanase that could be exploited as a potential source of feed industry.