WIST-2抑制慢性髓系白血病细胞株MEG-01增殖的机制研究
he mechanism of the suppressive role of TWIST-2 in chronic myeloid leukemic cell line MEG-01
目的 研究TWIST-2对慢性髓系白血病细胞株MEG-01体内、体外增殖的抑制作用及其机制。方法 利用慢病毒体系在MEG-01细胞中过表达TWIST-2，通过细胞生长曲线、集落生成能力和小鼠皮下成瘤能力实验检测TWIST-2对MEG-01体内与体外增殖的影响；并通过细胞周期测定和Western分别检测TWIST-2对MEG-01细胞水平和相关信号通路分子水平的影响。结果1）过表达TWIST-2后，MEG-01细胞体内外增殖和体内成瘤能力均受到明显抑制；2）TWIST-2对MEG-01细胞的抑制与发生G0/G1期阻滞相关；3）TWIST-2显著抑制BCR-ABL的表达、活性及其下游JAK2/STAT5的磷酸化水平。结论 TWIST-2可以抑制MEG-01细胞中BCR-ABL的表达和活性，并抑制其下游JAK2-STAT5信号通路，从而减缓细胞周期进程，并最终抑制MEG-01细胞的体外增殖及体内成瘤能力。
Objective. To study the effects of TWIST-2 on MEG-01 cells (CML cells) and its underlying mechanisms. Methods. Using lentivirus system to deliver TWIST-2 into MEG-01 cells, then proliferation assay, colony-forming cell assay and tumorigenesis injected subcutaneously in the nude mice were used to evaluate the effect of TWIST-2 overexpression in MEG-01 cells. Cell cycle analysis were used to study the cellular mechanism of TWIST-2 overexpression in MEG-01 cells. And western blot was to used to analyze the expression of BCR-ABL and its downstream components to reveal the molecular mechanism upon TWIST-2 overexpression. Results. Upon TWIST-2 overexpression, the growth of MEG-01 cells was restrained, CFC ability was decreased and TWIST-2 protected nude mice from tumorigenesis after injected subcutaneously. The cell cycle was arrested in G0/G1 stage, and the expression and activity of BCR-ABL was decreased, in addition JAK2-STAT5 signal was attenuated. Conclusion. TWIST-2 is able to inhibit the growth of MEG-01 cells possibly through the suppression of BCR-ABL/JAK2/STAT5 signaling.
肖伦、张秀艳、吴洁、马文娟、赵昀
肿瘤学基础医学分子生物学
白血病慢性髓系白血病MEG-01WIST-2
LeukemiaCMLMEG-01TWIST-2
肖伦,张秀艳,吴洁,马文娟,赵昀.WIST-2抑制慢性髓系白血病细胞株MEG-01增殖的机制研究[EB/OL].(2016-05-11)[2025-04-29].http://www.paper.edu.cn/releasepaper/content/201605-185.点此复制
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