Survivin在视网膜/脉络膜血管内皮细胞中的作用研究

Purpose: To evaluate the roles of survivin in the retinal/choroidal vascular endothelial cells (RF/6A). Methods: Immunofluorescence analyses were performed to determine the sites of survivin in RF/6A cells. siRNA technology was used to knock down survivin expression and to study its effects on cells in vitro. Cell proliferation, migration, spreading, cycling, and apoptosis were assessed with the MTT assay, Boyden chamber assay, immunocytochemistry, and flow cytometry. Tube formation by RF/6A on Matrigel was also analyzed. Results: Survivin was expressed in the nucleus in RF/6A cells in vitro. Survivin siRNA knockdown inhibited cell proliferation, migration. Under hypoxic conditions, more apoptotic cells were observed among the knockdown cells than among the control cells.Tube formation by RF/6A cells was also disturbed. Conclusions: Silencing the expression of survivin in RF/6A cells inhibited their proliferation and reduced their tolerance of hypoxic conditions. This study indicates that survivin may be a molecular target for new diagnostic and therapeutic strategies for Proliferative vitreoretinopathy diseases.