枇杷果实乙烯生物合成及相关基因表达研究

hree ethylene biosynthesis related genes, i.e. EjACS1, EjACO1, EjACO2 were cloned from non-climacteric loquat fruit (Eriobotrya japonica Lindl. cv. Luoyangqing). Real-time quantitative PCR (Q-PCR) analysis showed the fruit tissue specificity of EjACS1 and EjACO1 gene expression while higher expression of EjACO2 could be detected in leave and flower as well. Expression pattern of EjACO2 was consistent with the ethylene production during fruit development, reaching a peak when the fruit color was turning. EjACS1, EjACO1, and EjACO2 showed different expression patterns in fruit under different storage temperatures. It is the first time to study the gene expression of ethylene biosynthesis related genes in loquat fruit. Climacteric increases in ethylene production and respiration rate were observed during the development of loquat fruit and EjACO2 may play an important role in such a process.