Isolation and characterisation of watermelon ( Citrullus lanatus ) extracellular vesicles and their cargo
Isolation and characterisation of watermelon ( Citrullus lanatus ) extracellular vesicles and their cargo
Abstract Extracellular vesicles (EVs) facilitate cell-cell communication in animals and are integral to many physiological and pathological processes. Evidence for the presence and function of EVs in plants is limited. Here, we report that EVs derived from watermelon fruit mesocarp are of similar size and morphology to the animal EV subtype known as exosomes. Analysis of EV constituents revealed that watermelon EVs are negative for endoplasmic reticulum markers, and that the miRNA and protein profiles differ from that of watermelon mesocarp cells, suggesting that these EVs are actively synthesised and are not merely cellular debris. Furthermore, we report a panel of proteins found in in watermelon EVs as well as the published proteomes of grape, grapefruit, lemon and Arabidopsis thaliana EVs that are novel potential plant EV markers. Bioinformatic analyses suggest that plastids and multivesicular bodies are likely sites of biogenesis for EVs from watermelon and other plants. Predicted functional roles of watermelon EVs include development and metabolism, with several of their cargo molecules likely to be key in regulation of fruit development and ripening. Further understanding of how EVs may contribute to these processes would improve understanding of plant cell-cell communication and could aid in the harnessing of plant EVs for greater temporal control of crop development/ripening for the agricultural and retail industries.
Forbes Karen、Timms Kate、McLaughlin John、Holder Beth、Westwood Melissa、Day Anil
Maternal and Fetal Health Research Centre, School of Medical Sciences, University of Manchester, St Mary?ˉs Hospital||Discovery and Translational Science Department, Leeds Institute of Cardiovascular and Metabolic Medicine, Faculty of Medicine and Health, University of LeedsMaternal and Fetal Health Research Centre, School of Medical Sciences, University of Manchester, St Mary?ˉs Hospital||Manchester University NHS Foundation Trust, Manchester Academic Health Sciences Centre, St Mary?ˉs HospitalManchester University NHS Foundation Trust, Manchester Academic Health Sciences Centre, St Mary?ˉs Hospital||Division of Diabetes, Endocrinology and Gastroenterology, University of Manchester||Department of Gastroenterology, Salford Royal NHS Foundation TrustFaculty of Medicine, Department of Metabolism, Digestion and Reproduction, Imperial College LondonMaternal and Fetal Health Research Centre, School of Medical Sciences, University of Manchester, St Mary?ˉs Hospital||Manchester University NHS Foundation Trust, Manchester Academic Health Sciences Centre, St Mary?ˉs HospitalDivision of Molecular and Cellular Function, School of Biological Sciences, University of Manchester, St Mary?ˉs Hospital
植物学细胞生物学生物科学研究方法、生物科学研究技术
extracellular vesiclesexosomesplastidmultivesicular bodymiRNA
Forbes Karen,Timms Kate,McLaughlin John,Holder Beth,Westwood Melissa,Day Anil.Isolation and characterisation of watermelon ( Citrullus lanatus ) extracellular vesicles and their cargo[EB/OL].(2025-03-28)[2025-08-05].https://www.biorxiv.org/content/10.1101/791111.点此复制
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