胚脑宫内缺血缺氧时GSK-3β和β-Catenin mRNA的变化

Wnt-signalling pathway has pivotal roles during the development of embryo brian. Recent studies have implicated a role for the canonical WNT/β-catenin signal transduction in rat hypoxic-ischemia preconditioned myocardium. However, the relationship between Wnt pathway and intrauterine hypoxia-ischemia brain damage (HIBD) has not been described. We used a transient intrauterine model as embryo HIBD in gestational rat (14, 16, 18, 20days). The rats were randomly divided into two groups. For transient intrauterine ischemia group, the gestational rat’s bilateral uterine arteries were occluded for 30 min, followed by reperfusion. For sham operation, animals were subjected to the same surgical procedures without occlusion. Embryo brain samples were collected at 24 h during reperfusion. Results of HE staining and TUNEL staining confirmed that transient intrauterine hypoxia-ischemia significantly induced neuronal apoptosis. The Expression rate of TUNEL positive cells was significantly higher than sham operation group at each time point. Real Time PCR was used to evaluate the levels of GSK-3β and β-catenin mRNA. The difference of GSK-3β and β-catenin mRNA level was no statistical significance between two groups. This study suggested that the further research of the non-canonical Wnt/Ca2+ or Wnt/polarity signalling may contribute to a better understanding of Wnt signalling’s effect on HIBD.