电针对慢性应激抑郁大鼠海马激活素A和晚期糖基化终末产物受体表达的影响

Objective To explore the adjustment of electro-acupuncture on the expression of proteins of activin A (ACT A) and receptor for advanced glycation end products (RAGE) in the hippocampus of rats with chronic unpredictable mild stress-induced depression；To investigate the Anti-damage and growth promoting effect of electro-acupuncture on the the protection of hippocampal neurons. Methods 40 Sprague-Dawley rats were randomly divided into four groups: control group,model group,model＋Electro-acupuncture group (EA group),and model＋fluoxetine group (fluoxetine group). control group were raised for 28 days without any stimulation and treatment, the other groups were modeling by the chronic unpredictable mild stress (CUMS) combined with solitary raising methods to establish rat depression model for 28 days.EA group was given acupuncture on acupoints Baihui (DU 20) and Yintang (DU 29) for twenty minutes(2 Hz,0.6 mA)before modeling.Fluoxetine group was given fluoxetine at the dosage of 5 mlokg-1 before modeling. The expression of proteins of Act A and RAGE in hippocampus of rats were detected by biotin label-based antibody array.Results Compared to the control group,the expression of protein of Act A and RAGE ware both upregulation (fold change＝1.240,1.356) in the model group. Compared to the model group, the expression of protein of Act A and RAGE ware downregulation (fold change＝0.701,0.604; fold change＝0.617,0.624).conclusion The treatment of electro-acupuncture could down- regulate the protein expression level of RAGE in order to resist damage damage and benign-regulate the protein expression level of Activin A to normal level in the hippocampus of rats with chronic unpredictable mild stress-induced depression, which may be involved in promoting the regeneration of neurons. All of it may be one of the molecular mechanism of antidepressant effect of electro-acupuncture.