miR-24抑制BCL2L11表达促进胰腺癌生长的机制研究
he mechanism of miR-24 promoting tumor growth by targeting BCL2L11 in gastric cancer
目的:旨在研究BCL2L11表达异常在胰腺癌发病中的调控机制，并探索miRNA-BCL2L11信号通路对胰腺癌增殖、凋亡等生物学行为的影响。方法:通过生物信息学软件筛选出BCL2L11可能是miR-24的靶基因，利用荧光素酶报告基因验证miR-24与BCL2L11的调控关系，裸鼠腋下植瘤（Panc-1细胞过表达BCL2L11组、过表达miR-24组、对照组），4周后处死小鼠，取出肿瘤。测量肿瘤大小及重量，检测组织中BCL2L11及 miR-24表达水平。及对血管生成的调控。结果：荧光素酶报告基因结果显示miR-24可直接与BCL2L11的3'UTR区结合，miR-24过表达组的肿瘤生长较快，并且其BCL2L11蛋白表达水平明显下降，但mRNA水平无明显变化。MiR-24可以通过抑制BCL2L11的表达抑制体内肿瘤生长。结论：证实miR-24通过抑制BCL2L11表达调控胰腺癌细胞的增殖和凋亡。
his study aimed to explore the expression of BCL2L11 in Pancreatic cancer，select miRNAs which directly target BCL2L11 and further discuss the role of the miRNA-BCL2L11 pathway in the tumorigenesis ofin PaC. By using bioinformatics tools, we found that miR-24 can directly target the 3'UTR of BCL2L11 mRNA. Panc1 cells treated with control lentivirus or miR-24 overexpressing lentivirus or BCL2L11 overexpressing lentivirus were injected subcutaneously into nude mice. Mice were sacrificed after 4 weeks, and the weight and diameter of tumors were recorded. The expression of BCL2L11 and miR-24 were determined thereafter. ?????
刘锐、巴一
肿瘤学基础医学分子生物学
肿瘤学miR-24BCL2L11增殖凋亡 ?????
OcologymiR-24BCL2L11Proliferationapoptosis ?????
刘锐,巴一.miR-24抑制BCL2L11表达促进胰腺癌生长的机制研究[EB/OL].(2016-05-26)[2025-08-10].http://www.paper.edu.cn/releasepaper/content/201605-1264.点此复制
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