分离测定鱼腥草和山楂果实中槲皮素、芦丁和绿原酸反向迁移胶束毛细管电泳新方法

A reverse-flow micellar electrokinetic chromatographic (RF-MEKC) method with direct on-column UV detection was developed for the separation and determination of quercetin, rutin and chlorogenic acid in herba houttuyniae and hawthorn fruit. The separation conditions of the three analytes were optimized with respect to the concentrations of SDS, NaH2PO4, pH and applied voltage. Baseline separation was obtained for the three analytes within 15 min, using a running buffer containing 70 mM SDS, 30 mM NaH2PO4, pH 2.1 and −15 kV voltage. The linear calibration range was 25-400 µg/mL for quercetin and rutin; 12.5-200 µg/mL for chlorogenic acid (r＝0.9992、r＝0.9992、r＝0.9999). The recoveries were 98.5%~103.7%， 95.8%~97.6% and 99.1~103.8% for quercetin, rutin and chlorogenic acid, respectively. The detection limits of three analytes (S/N=3) were 2.52、3.1、3.64 μg/mL, respectively.