雌激素对血清剥夺培养的心肌细胞的影响

Object] To study the effects of estrogen on the cell viability of cardiomyocytes cultured with serum deprivation. [Methods] The cell viability of cardiomyocytes was determined by MTT assay and the protein expression was determined by western blot. [Results] Cell culture with serum deprivation decreased cell viability of cardiomyocytes and increased cell death. Estrogen treatment aggravated serum deprivation-induced cardiomyocyte death in a dose-dependent manner. Estrogen showed no effect on cardiomyocyte viability in normal culture conditions. Serum deprivation for different time induced increase of LC3II expression, indicating that serum deprivation induced increase of cardiomyocyte autophagy. Estrogen treatment inhibited the autophagy response induced by serum deprivation. Rapamycin treatment induced increase of cardiomyocyte autophagy, but rapamycin did not alleviate the effect of estrogen-aggravated serum deprivation-induced cardiomyocyte death. The level of p-smad1/5/8 was decreased in cultured cardiomyocytes with different serum deprivation. Bone morphogenetic protein-2 (BMP2) alleviated serum deprivation-induced decrease of p-smad1/5/8 expression and alleviated serum deprivation-induced decrease of cell viability. [Conclusions] Estrogen aggravated serum deprivation-induced cardiomyocyte death; Bone morphogenetic protein-2 alleviated estrogen-aggravated serum deprivation-induced cardiomyocyte death.