松杨栅锈菌地理菌群的SCAR标记转换

his paper focused on achieving sequence characterized amplified region(SCARs) of geographic population of Melampsora larici-populina. .Based on the results of RAPD study of Melampsora larici-populina，DNA fragments of Western and Northern populations were recycled, purified, pGEM-T lector cloned and sequenced. About 400bp length DNA fragments were achieved after being integrated by software Sequmen, and two pairs of primers were designed by software Primerselect (Western population primers: PNW0118-382-F: GAATCGGCCACAAAACTATC, PNW0118-382-R: GAATCGGCCATGACTTGAGC,and Northern population primers: PNW0118-400-F: GAATCGGCCACAAAACTATC,PNW0118-400-R:GAATCGGCCATGACTTGAGCTGC). With these two pairs primers, SCARs of Western population (382bp) and Northern population (400bp) were got successfully by Polymerase Chain Reactions.