荧光猝灭法测定苏丹红I的方法研究

fter using fluorescence Spectrophotometer to measure Sudan I, the reseults show as follow: when the Tryptophan is combined with the Sudan I, the fluorenscence in the Tryptophan will be quenched. Withing a certain range of 0.0014—0.0095ug/mL（R=0.9984）, the fluorenscence quenching effects has a linear character of relationship with the thickness of Sudan I—acetonitrile, based on this situation a method of determining the Sudan I content quickly in the chili with the adoption of using the Tryptophan Fluorenscence Quenching process has been established. The detection limit of this method is 0.0007 ug/mL, while the method of using the Fluorenscence Queching process to determine the Sudan I is original, fast, simple, with high delicacy and selectivity and requiring less resource. It is used to determine the Sudan I content in the Tine chili and the GanGu chili (a kind of chili with slim and very long shape),the result’s satisfied.