红枝垂组织培养快繁技术的研究

Selected explants, stem section with bud,were inoculated medium of MS 1.0 mg /L N6 -benzyladenine (BA) and 0.1mg/L α-naphthaleneacetic acid (NAA) , the highest shoot induction rate is 80%. The N6 -benzyladenine (BA), α-naphthaleneacetic acid (NAA) and sucrose were used to compare their effect on shoot proliferation coefficient in the C. subhirtella var. pendula ‘Rosea’ by using the orthogonal experiment design. The test result indicated that cytokinins(BA) was the main factor. The optical medium for reproduction of the bud was A2B3C2, namely, MS with 0.5 mg /L BA and 0.1 mg /L NAA + 35 g/L sucrose; Proliferation coefficient was 4.15. Plantets were transplanted the 1/2MS medium with 1.0 mg/L indole-3-btyricacid (IBA) and 1.0mg/L α-naphthaleneacetic acid (NAA) for rooting, when the explants were reached 2-3cm.,the rooting rate could reach 95% after 25 days. Opening the culture bottle during seedling training in 5-7d, Substrate with sand, perlite and vermiculite by 1 to 1 to 1 was the optimal. After 20 days the survival rate of transplants reached 84%.