蒙古羊HOX基因片段的克隆及序列分析
loning and Sequences Analysis of Homeobox Gene Fragments
同源异型基因都含有一个共同的序列，称为同源异型框，它编码一个同源异型蛋白，称之为同源异型域，同源域蛋白是一类主要的转录调节因子,通过该结构与DNA特异的核苷酸序列结合,实现对靶基因的调控。为探讨同源盒(HOX)基因在蒙古羊毛囊生长发育与细胞分化中的表达情况，选用已报道的Hox基因序列保守区序列设计一对简并引物，对蒙古羊的基因组进行了PCR扩增，经克隆和DNA测序，得到的片段为117bp，编码39个氨基酸，通过DNAStar软件及GenBank中Blastn分析证明克隆到了3个Hox基因片段。
Homeotic genes share a characteristic DNA segment, the homeobox, which encodes a defined domain of the homeotic protein-homeodomain which seems to mediate the binding to specific DNA sequences,whereby the homeotic protein exerts a gene regulatory function. To investigate the expression of Mongolia sheep homeobox(Hox) genes in hair follicle development and growth, the degenerate primers are designed on the known conserved sequence of homeobox genes, the homeobox gene fragments are amplified by PCR, then it be clone and sequence. These fragments are 117 bp, encodes 39 amino acids, the sequences are analyzed by DNAStar5.01 and Blastn indicate that three sheep Hox fragments are cloned.
姜斌、吴江鸿 、吴广宇、尹俊、严淑兰
遗传学分子生物学动物学
蒙古羊Hox基因克隆
Mongolia sheepHomeobox(HOX) genesGene clone
姜斌,吴江鸿 ,吴广宇,尹俊,严淑兰.蒙古羊HOX基因片段的克隆及序列分析[EB/OL].(2009-01-16)[2025-05-16].http://www.paper.edu.cn/releasepaper/content/200901-840.点此复制
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