小鼠皮肤切创愈合过程中CB1受体的变化规律及其与损伤时间相关性的研究

Objective To investigate the change of cannabinoid receptor type 1 (CB1R) during the the skin incised wound healing in mice and to explore the rule of the time-dependent change of CB1R in wound age determination. Methods The changes of CB1 expression in incised skin wound were detected by immunohistochemistry and Western blot. Results There was a minimal baseline staining of CB1 in control mouse skin，which is expressed in epidermis, hair follicles, sebaceous gland, muscular layer. Changes of CB1 expression were undetectable in neutrophils in the wound specimens from 6 hours to 12 hours after injury. The CB1 positive cells were mostly mononuclear cells and fibroblasts between post-injury day 1 and 5, whereas the CB1 postive cells were mostly fibroblasts between post-injury day 7 and day 14. Morphometrically, the ratio of the CB1 positive cells to the total increased gradually in the wound specimens from 6h to day 3, maximized at day 5, and then decreased guadually from post-injury day 7 to day 14. The changes of CB1 expression were observed throughout the wound healing stages by Western blot as well, with a peak in specimens of day 5. Conclusion CB1 was activated during the wound healing.It is partly expressed in mononeuclear cells, which may be involved in inflammation. CB1 was observed in fibroblasts, which may participate in wound healing, and meanwhile, CB1 may be a potentially useful marker for wound age determination.