不同时程铅暴露对海马神经元AMPA受体表达的影响

Environmental lead damage the function of learning and memory, but the mechanism of lead influence subtypes of AMPA receptor expression is unclear. This research used primary cultured hippocampal neurons and adopted electrophysiological, immunocytochemistry and methods of molecular biology to investigate the effects of different time lead exposure on synaptic basic transmission and AMPA receptor expression. The results found that amplitude and frequency of miniature excitatory postsynaptic currents had no significant changes after neurons respectively lead exposure for 5 minutes, 15 minutes, 4 hours and 24 hours; Total protein expression of AMPA receptor subunits GluR1 and GluR2 had no obvious changes after lead exposure for 5 minutes, 15 minutes, 4 hours. But the protein expression of excitatory postsynaptic membrane GluR1 increased significantly after lead exposure for 15 minutes. In conclusion, 15 minutes of acute lead exposure can cause GluR1 synaptic membrane trafficking efficiency.