Pediatric Multi-Organ Dysfunction Syndrome: Analysis by an Untargeted “Shotgun” Lipidomic Approach Reveals Low-abundance Plasma Phospholipids and Dynamic Recovery Over 8-Day Period, a Single-Center Observational Study
Pediatric Multi-Organ Dysfunction Syndrome: Analysis by an Untargeted “Shotgun” Lipidomic Approach Reveals Low-abundance Plasma Phospholipids and Dynamic Recovery Over 8-Day Period, a Single-Center Observational Study
Abstract Lipids are stable molecules involved in metabolism and inflammation. We investigated the plasma lipidome for markers of severity and nutritional status in critically ill children. Children with multi-organ dysfunction syndrome (MODS) (n=24) were analyzed at three time points and cross referenced to sedation controls (n = 4) for a total of N=28. Eight of the patients with MODS, needed veno-arterial extracorporeal membrane oxygenation (VA ECMO) support to survive. Blood plasma lipid profiles were quantified by nano-electrospray (nESI), direct infusion high resolution/accurate mass spectrometry (MS), and tandem mass spectrometry (MS/MS) and compared to nutritional profiles and PEdiatric Logistic Organ Dysfunction (PELOD) scores. PELOD scores were not significantly different between MODS and ECMO cases across time-points (p = 0.66). Lipid profiling provided stratification between sedation controls and all MODS patients for lysophosphatidylserine (lysoPS) (p-value = 0.004), total phosphatidylserine (PS) (p-value = 0.015), and total ether-linked phosphatidylethanolamine (PE) (p-value = 0.03). Phospholipids in patients needing ECMO were observably closer to sedation controls than other MODS patients. Nutrition intake revealed changes in lipid profiles that corresponded to calorie and protein intake. Lipid measurement in the intensive care environment shows dynamic changes over an 8-day PICU course, suggesting novel indicators for defining critically ill children.
Sanfilippo Dominic、Boville Brian、Wolfrum Emily、Prokop Jeremy W.、Rajasekaran Surender、Ferguson Karen、Lydic Todd A.、Leimanis Laurens Mara L.
Pediatric Critical Care Unit, Helen DeVos Children?ˉs Hospital||Department of Pediatric and Human Development, College of Human Medicine, Michigan State University, Life Sciences Bldg. 1355 Bogue StreetPediatric Critical Care Unit, Helen DeVos Children?ˉs Hospital||Department of Pediatric and Human Development, College of Human Medicine, Michigan State University, Life Sciences Bldg. 1355 Bogue StreetVan Andel Institute, Bioinformatics & Biostatistics CoreDepartment of Pediatric and Human Development, College of Human Medicine, Michigan State University, Life Sciences Bldg. 1355 Bogue Street||Department of Pharmacology and Toxicology, Michigan State UniversityPediatric Critical Care Unit, Helen DeVos Children?ˉs Hospital||Department of Pediatric and Human Development, College of Human Medicine, Michigan State University, Life Sciences Bldg. 1355 Bogue StreetPediatric Critical Care Unit, Helen DeVos Children?ˉs HospitalDepartment of Physiology, Collaborative Mass Spectrometry CorePediatric Critical Care Unit, Helen DeVos Children?ˉs Hospital||Department of Pediatric and Human Development, College of Human Medicine, Michigan State University, Life Sciences Bldg. 1355 Bogue Street
儿科学基础医学医学研究方法
lipidomicspediatricscritical illnessmulti-organ dysfunction syndromephospholipidsnutritional intake
Sanfilippo Dominic,Boville Brian,Wolfrum Emily,Prokop Jeremy W.,Rajasekaran Surender,Ferguson Karen,Lydic Todd A.,Leimanis Laurens Mara L..Pediatric Multi-Organ Dysfunction Syndrome: Analysis by an Untargeted “Shotgun” Lipidomic Approach Reveals Low-abundance Plasma Phospholipids and Dynamic Recovery Over 8-Day Period, a Single-Center Observational Study[EB/OL].(2025-03-28)[2025-06-29].https://www.medrxiv.org/content/10.1101/2020.11.24.20237891.点此复制
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