神经激肽受体2的拮抗剂SR48968对三种白血病细胞的作用及其作用机制的初步探讨

Previous studies showed that the expression levels of the neurokinin receptos were changed in some tumors or cancer cells. SR48968 is an antagonist of the neurokinin receptor 2. Leukaemia is a type a disease which have serious harm to human health. In this study, the human chronic myelogenous leukemia cells K562, the human acute myeloid leukemia cells HL-60 and the murine erythroleukemia cells MELwere used to investigate the effects of SR48968 to the three leukaemia cells. The results of MTT test and optical microscopy observation showed that SR48968 inhibited the growth of the three types of cells in a dose- and time-dependent manner. Moreover, the inhibited effects of the growth of leukaemia cells induced by SR48968 may be mediated through an apoptosis pathway, which was obtained from the Hoechst 33342 staining test. Additionally, the data from two dimensional electrophoresis（2-DE）showed that the expression levels of more than 100 proteins were changed after administrated SR48968.The results of this study will be helpful to the further study between the neurokinin receptors and the occurrence and development of tumors, and the future follow-up studies in depth will provide new ideas and potential drugs in diagnosis and treatment of clinical tumors.