复方浙贝颗粒联合阿霉素对P388移植瘤p53基因表达影响

Objective Studying the impact on p53 gene expression which is caused by compound zhe-bei granule and adriamycin in the P388 xenograft tumor. Methods Establishing tumor xenografts model by injecting the mouse lymphocytic leukemia cells (P388) in the subcutis of anterior axillary of Kunming mice, then treating with CZBG by intragastric administration and different doses of adriamycin by intraperitoneal injection (i.p.). After the end of the experiment, striping tumor completely, detecting the expression of p53 gene in xenograft tumor of each group by fluorescence quantitative polymerase chain reaction, and computing the relative quantification of p53 gene of each group using 2-ΔΔCt method. Results Comparing the 2-ΔΔCt values of p53 gene relative expression of each group, no statistical significance is found (F=0.56, P=0.7557). And the relative expression value of p53 gene of CZBG Joint high-dose ADM group is higher, while the relative expression value of CZBG Joint low-dose ADM group is lower. Conclusion Combined use of CZBG and ADM seems to be able to raise the expression of p53 tumor suppressor gene in the P388 xenograft tumor.