锈腐病人参对MCF-7细胞的抑制作用及机制研究

Objective The purpose of this article is to study the inhibitory effect of ginseng rust rot on the proliferation of MCF-7 breast cancer cells and its mechanism. Methods The cell morphology and mechanical characteristics of the MCF-7 cells treated with different concentrations of ginseng rust rot were investigated by SEM (scanning electron microscopy) and AFM (atomic force microscopy), respectively. The plate clone formation experiment was used to test the clonal formation abilities in vitro. The expression of β-actin in MCF-7 cells was detected by Western blot. Results Compared with the control cells, both the morphology and mechanical properties of MCF-7 cells changed greatly after treated with the extract of ginseng rust rot. These changes were dose dependent. With the increased concentration of ginseng rust rot extract, more proteins were observed on the surface of MCF-7 cells. The distance between cells became larger. The cell average height gradually increased, while the average adhesion and the average Young\'s modulus declined, especially for the decrease of 2.37 kPa in the average Young\'s modulus. In addition, the cloning ability of MCF-7 cells was significantly inhibited, and the expression of β-actin protein was also significantly reduced. Conclusion Ginseng rust rot can inhibit the MCF-7 cell proliferation, and it may be related to the decline of intracellular β-actin.?