构巢曲霉中YvcA与CnaA/CchA协同调控真菌对盐逆境的响应

In budding yeast, hypertonic shock induces the release of calcium from internal stores through Yvc1p. However, the mechanisms of coordinating regulation of YvcA and calcineurin/CchA in response to salt stress are not well understood. The loss of cnaA caused hyphal growth and conidiation defects under salt stress. The deletion of cchA suppresses the hyphal growth defects caused by the loss of calcineurin under salt stress. Although the deletion of yvcA does not affect the hyphal radial growth and conidiation when grown on the presence or absence of the Ca2+ chelator EGTA or EGTA plus NaCl in Aspergillus nidulans, YvcA was proved to compensate with calcineurin-CchA in fungal salt-stress adaption. Further, Using the calcium-sensitive photoprotein aequorin to test the [Ca2+]c in living cells, we found that CnaA and CchA synergistically coordinate calcium influx under salt stress. Collectively, the results provide insights into new linkages between YvcA and CnaA/CchA in the regulation of cell salt-stress survival processes.????