新型胶原支架材料的结构特征与性能研究

In order to overcome the shortcomings of acellular dermal matrix which is used as tissue engineering scaffold material, such as bad permeability, low degradation speed, strong immunogenicity and so on, a new type of collagen scaffolds were prepared by adopting various chemical, biological and physical comprehensive methods to deal with pigskin. The microstructure of the scaffolds was observed under photomicroscope and scanning electron microscopy (SEM) and the vitro degradation time, permeability, tensile-strength, porosity, shrinkage temperature were studied. Experimental results show: its dermal fibroblasts, lipocytes and interstitial substances between collagen fibers have been removed completely, simultaneously collagen fibers have been appropriately loosen, and the original natural three dimensional meshwork porous structure is maintained；The permeability is 3000g•m-2•24h-1around, which is fit for wound healing; The degradation time is between 25h and 50h, and can be controlled by adjusting technological conditions; The tensile-strength is between 10.20MPa and 11.50MPa which is better；The shrinkage temperature is between 70℃ and 85℃. All of this indicate this materials have overcame the shortcomings of acellular dermal matrix, and have high permeability, tensile-strength, proper degradation speed. So it conforms to the tissue engineering scaffold material requirements.