pEGFP-N1/BMP-2真核表达质粒的构建与鉴定
onstruction and identification of eukaryotic expression vector
目的 构建pEGFP-N1/BMP-2真核表达质粒，并进行鉴定。方法 利用RT-PCR方法从组织中提取骨形态发生蛋白（BMP-2）的基因片段，与pMD18-T载体连接，构建pMD18-T/BMP-2重组质粒，酶切后与pEGFP-N1真核表达载体连接，构建pEGFP-N1/BMP-2真核表达质粒，进行测序、酶切鉴定，表明质粒构建成功。结果 本实验成功构建了pEGFP-N1/BMP-2真核表达质粒，为进一步研究利用BMP-2基因修饰骨组织工程骨种子细胞提供实验基础。
Objective To Construct a eukaryotic expression vector carrying BMP-2 gene.Methods BMP-2 gene was cloned from tissue by using RT-PCR method and was inserted into pMD18-T to constrcuct pMD18-T/BMP-2 plasmid,and to construct pEGFP-N1/BMP-2 plasmid by using restriction enzyme.To identify the plasmid by sequencing and restriction enzyme.Result The eukaryotic expression vector pEGFP-N1/BMP-2 was constructed and identified.
刘光耀、柳扬、高忠礼、常非、张明磊
分子生物学生物工程学
pEGFP-N1骨形成蛋白2基因
pEGFP-N1BMP-2gene
刘光耀,柳扬,高忠礼,常非,张明磊.pEGFP-N1/BMP-2真核表达质粒的构建与鉴定[EB/OL].(2010-01-06)[2025-08-18].http://www.paper.edu.cn/releasepaper/content/201001-208.点此复制
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