电压门控质子通道Hvl提高乳腺癌细胞迁移和侵袭的潜能

Objective: Clarify the effect of Voltage-gated proton channel（Hv1） on the migration and invasion of breast cancer cells. Methods: The protein expression of Hv1were detected in human breast cancer cell lines with different metastatic ability. SiRNA technique was used to down-regulate the expression of Hvl in breast cancer MDA-MB-231 cells, scratch and matrigel invasion methods were used to observe the effect of Hvl on migration and invasion of breast cancer cells, and the relevant molecular mechanism were explored. Results: Hv1 is highly expressed in the highly metastatic breast cancer MDA-MB-231 cells. Hvl was expressed higher in the MDA-MB-231 cells with high metastatic ability. The SiRNA sequence target at Hvl could inhibit the expression of Hvl. Scratch and matrigel invasion experiments showed that the migration and invasion of MDA-MB-231 cells were significantly attenuated when Hv1 was knocked down by siRNA targeting to Hv1 and the result of Zymography experiment of matrix metalloproteinase indicated that the enzyme activities of MMP-2 was markedly decreased. Conclusion: Hv1 promotes the migration and invasion ability of breast cancer cells.