长角血蜱专性共生菌CLS-Hl的数量与动态

Symbiont is a kind of microorganism which forms a symbiotic relationship with organism. Symbiont are ubiquitous in a varity of insect hosts and play multiple and important roles in their hosts, such as, giving assistance for nutrition metabolism of hosts, avoiding natural enemies, improving adaptability and so on. However, the studies of tick symbiont are much less than insect symbiont. Previously, we found that obligate symbiont CLS-Hl existed in H. longicornis and could be stably transmitted in the laboratory-reared tickss. Hence, we investigated the density and dynamics of CLS-Hl during the growth and development of H. longicornis by real-time quantitive PCR (qPCR). Results revealed that CLS-Hl suffered bottleneck effect in vertical transmission and the density of CLS-Hl fluctuated with the development of ovary, suggesting that this symbiont was associated with the reproduction fitness of H. longicornis and might provide some essential nutritions or regulatory factors for the development of ovary. Therefore, this study can lay the foundation for researching the function of CLS-Hl and the mutualistic mechanisms between symbionts and tick hosts, even provide some theory basis for inventing some new control technology.