成釉细胞瘤细胞的原代培养
primary culture of ameloblastoma cells
目的：探索成釉细胞瘤细胞生长特性，挑选适合实验的细胞。方法：原代培养成釉细胞瘤细胞，观察其形态及生长情况，作特异性抗体检测。结果：原代培养成釉细胞瘤细胞，可以保持肿瘤形态三代，持续57天，免疫组化显示角蛋白18、19，bcl-2表达阳性，波形蛋白表达阴性。结论：第二代细胞量较多，细胞形态变化不大，比较适合进行实验研究。
Objectives: To explore the characters of AM cells and select the most suit for experiment. Methods：AM cells was primary culture and observed the cells' morphous and growth states, and detected with specific antibody. Results: AM cells in primary culture maintained epithelial cell morphology and expressed cytokeratins K18 and K19, but none expressed vimentin. Furthermore, bcl-2 protein, which prevents apoptosis, was expressed. AM cells underwent senescent within fifty-seven days. Conclusion: the second filial generation is most suit for experiment.
孙钢
基础医学细胞生物学肿瘤学
成釉细胞瘤细胞培养免疫组化
ameloblastomacell culturehistochemistry
孙钢.成釉细胞瘤细胞的原代培养[EB/OL].(2011-02-16)[2025-08-02].http://www.paper.edu.cn/releasepaper/content/201102-168.点此复制
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