匹罗卡品致痫小鼠海马EphA4蛋白动态表达变化

In this paper we studied the dynamic expression pattern of EphA4 protein in the hippocampus of a mouse model of temporal lobe epilepsy induced by pilocarpine. Forty-eight SPF C57BL / 6 mice were randomly divided into control group and status epilepticus (SE) group. The SE group was subdivided into three groups according to the duration of the behavioural features, respectively in the acute period (7 days), the latent period (14 days) and the chronic period (28 days). SE was induced by intraperitoneal injection of pilocarpine. Immunohistochemical staining was used to detect the expression of EphA4 protein in the hippocampus at different time points after SE. EphA4 protein was mainly distributed in the granule cell layer of dentate gyrus, subgranular zone, hilus, pyramidal cell layer of CA1 and CA3 areas. The expression EphA4 protein gradually increased and peaked at 28 days post SE. There was significant difference between SE group and control group at 28 days (p < 0.001). No significant difference between the control groups. The upregulation of EphA4 protein expression indicated that EphA4 may be involved in the formation of abnormal neuronal network.