Metabolomic, photoprotective, and photosynthetic acclimatory responses to post-flowering drought in sorghum
Metabolomic, photoprotective, and photosynthetic acclimatory responses to post-flowering drought in sorghum
Abstract Climate change is globally affecting rainfall patterns, necessitating the improvement of drought tolerance in crops. Sorghum bicolor is a drought-tolerant cereal capable of producing high yields under water scarcity conditions. Functional stay-green sorghum genotypes can maintain green leaf area and efficient grain filling in terminal post-flowering water deprivation, a period of ~10 weeks. To obtain molecular insights into these characteristics, two drought-tolerant genotypes, BTx642 and RTx430, were grown in control and terminal post-flowering drought field plots in the Central Valley of California. Photosynthetic, photoprotective, water dynamics, and biomass traits were quantified and correlated with metabolomic data collected from leaves, stems, and roots at multiple timepoints during drought. Physiological and metabolomic data was then compared to longitudinal RNA sequencing data collected from these two genotypes. The metabolic response to drought highlights the uniqueness of the post-flowering drought acclimation relative to pre-flowering drought. The functional stay-green genotype BTx642 specifically induced photoprotective responses in post-flowering drought supporting a putative role for photoprotection in the molecular basis of the functional stay-green trait. Specific genes are highlighted that may contribute to post-flowering drought tolerance and that can be targeted in crops to maximize yields under limited water input conditions. HighlightPathways contributing to the long-term maintenance of photosynthetic activity in terminal post-flowering drought are revealed by a comprehensive approach combining in-field photosynthetic physiological analysis, metabolomics, and transcriptomics.
Patel Dhruv、Cole Benjamin J.、Dautermann Oliver、Kelikian Armen C.、Bilbao Aivett、Kyle Jennifer E.、Bloodsworth Kent J.、Paurus Vanessa、Sievert Julie、Dahlberg Jeffery、Ching Lindsey G.、Hutmacher Robert、Hixson Kim K.、Kim Young-Mo、Pedraza Julie、Lemaux Peggy G.、Baker Christopher R.、Lee Joon-Yong、Niyogi Krishna K.、Allison Cayci
Dept. of Plant and Microbial Biology, University of CaliforniaDOE-Joint Genome Institute, Lawrence Berkeley National LaboratoryDept. of Plant and Microbial Biology, University of CaliforniaDept. of Plant and Microbial Biology, University of CaliforniaEnvironmental Molecular Sciences Laboratory, Pacific Northwest National LaboratoryBiological Sciences Division, Pacific Northwest National LaboratoryBiological Sciences Division, Pacific Northwest National LaboratoryBiological Sciences Division, Pacific Northwest National LaboratoryUC-ANR Kearney Agricultural Research and Extension (KARE) CenterUC-ANR Kearney Agricultural Research and Extension (KARE) CenterDept. of Plant and Microbial Biology, University of CaliforniaDepartment of Plant Sciences, University of CaliforniaEnvironmental Molecular Sciences Laboratory, Pacific Northwest National LaboratoryBiological Sciences Division, Pacific Northwest National LaboratoryUC-ANR Kearney Agricultural Research and Extension (KARE) CenterDept. of Plant and Microbial Biology, University of CaliforniaHoward Hughes Medical Institute, Dept. of Plant and Microbial Biology, University of CaliforniaBiological Sciences Division, Pacific Northwest National LaboratoryHoward Hughes Medical Institute, Dept. of Plant and Microbial Biology, University of California||Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National LaboratoryUC-ANR Kearney Agricultural Research and Extension (KARE) Center
农业科学研究植物学生物化学
Sorghum bicolorphotosynthesisdrought tolerancephotoprotectionmetabolomicsantioxidantsstomatal closurestay-green
Patel Dhruv,Cole Benjamin J.,Dautermann Oliver,Kelikian Armen C.,Bilbao Aivett,Kyle Jennifer E.,Bloodsworth Kent J.,Paurus Vanessa,Sievert Julie,Dahlberg Jeffery,Ching Lindsey G.,Hutmacher Robert,Hixson Kim K.,Kim Young-Mo,Pedraza Julie,Lemaux Peggy G.,Baker Christopher R.,Lee Joon-Yong,Niyogi Krishna K.,Allison Cayci.Metabolomic, photoprotective, and photosynthetic acclimatory responses to post-flowering drought in sorghum[EB/OL].(2025-03-28)[2025-07-16].https://www.biorxiv.org/content/10.1101/2022.01.14.476420.点此复制
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