成骨细胞复合β-磷酸三钙异位成骨的实验研究

objective: The present study was carried out to evaluate the construct combined osteoblasts with β-tricalcium phosphate (β-TCP) as bone substitute implanted in rat dorsal muscles. Methods: To study the osteogenic capability of in vivo, specimens were harvested on 1 week, 4 weeks and 8 weeks after implantation, and were analyzed by hematoxylin and eosin (HE) staining. The percentages of new bone formation for each implant type and implantation period were determined by histomorphometry. Results: After 1 week of implantation, new bone formation for both β-TCP and osteoblasts+β-TCP group had no formed. At 4 weeks, the amount of bone formation was increased to 1.32 % in β-TCP group and 6.35% in osteoblasts+β-TCP group. After 8 weeks of implantation, more bone was found in the osteoblasts+β-TCP group (21.58 %), while in the β-TCP group bone formation was increased to 4.78%. Significant differences between the two groups have been observed. Conclusions: Based on these results, we conclude that bone substitutes constructed by porous β-TCP scaffold loaded with osteoblasts could promote newly formed bone.