miR-135b通过靶向FOXO1促进子宫内膜癌细胞的增殖
沈君菁 李学农 刘国炳 秦逸飞 黄启涛 乐珍
作者信息
摘要
目的 探讨miR-135b在子宫内膜癌中的表达及对子宫内膜癌细胞增殖的作用的机制。方法 运用RT-PCR的方法检测22对子宫内膜癌组织及相应的癌旁组织中的 miR-135b和 FOXO1的表达；运用 RT-PCR的方法检测 JEC、Ishikawa、HEC-1-B、RL-952这4种子宫内膜癌细胞株中miR-135b和FOXO1的表达。分别转染miR-135b的模拟物抑制物于子宫内膜癌细胞株，通过RT-PCR检测转染的效果，通过MTT增殖试验检测对子宫内膜癌细胞增殖的影响。通过RT-PCR的方法检测FOXO1 mRNA的变化，Western blotting检测在 FOXO1 蛋白的变化。结果 miR-135b在子宫内膜癌组织中较对应的癌旁组织表达增高（P<0.05）, FOXO1在子宫内膜癌组织中较对应的癌旁组织表达降低（P<0.05）。荧光定量PCR显示子宫内膜癌细胞中miR-135b与FOXO1表达趋势成负相关。miR-135b能促进子宫内膜癌细胞增殖（P<0.05）。上调miR-135b能够降低FOXO1 mRNA和蛋白的表达（P<0.05），下调miR-135b能够提高FOXO1 mRNA和蛋白的表达（P<0.05）。结论 miR-135b在子宫内膜癌的发生发展中发挥重要的作用，其部分分子机制可能是通过调控靶基因FOXO1而发挥作用。Abstract
Objective To explore the expression of miR-135b in endometrial carcinoma and the mechanism by which miR-135b promotes the proliferation of endometrial cancer cells. Methods The expressions of miR-135b and FOXO1 were using RT-PCR detected in 22 fresh endometrial cancer tissues and paired adjacent tissues and also in endometrial cancer cell lines JEC, Ishikawa, HEC-1-B, and RL-952. The RL-952 and Ishikawa cell lines were transfected with miR-135b mimics or inhibitors, and the changes in their proliferative activity were detected with MTT assay; the expressions of FOXO1 mRNA and protein were detected by RT-PCR and Western blotting, respectively. Results The expression of miRNA135b was significantly up-regulated and FOXO1 expression was down-regulated in endometrial carcinoma tissues as compared with the adjacent tissues (P<0.05). The mRNA expression of miR-135b was negatively correlated with the expression of FOXO1 in endometrial carcinoma. In RL-952 and Ishikawa cell lines, transfection with miR-135b mimics obviously promoted the cell proliferation (P<0.05). Up-regulation of miR-135b significantly decreased the expressions of FOXO1 protein and mRNA (P<0.05), and downregulation of miR-135b increased FOXO1 expressions (P<0.05). Conclusions MiR-135b plays an important role in the occurrence and development of endometrial carcinoma partially by regulating its target gene FOXO1. 关键词
子宫内膜癌/增殖/FOXO1/ miR-135b引用本文复制引用
沈君菁,李学农,刘国炳,秦逸飞,黄启涛,乐珍.miR-135b通过靶向FOXO1促进子宫内膜癌细胞的增殖[EB/OL].(2017-12-27)[2025-12-14].https://chinaxiv.org/abs/201712.00968.学科分类
妇产科学/肿瘤学/基础医学
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