水通道蛋白5在大鼠大脑组织中的分布及表达

Objective To observe the distribution of aquaporin 5(AQP5) in rat brain, so as to provide the morphological base for studying the water transport in brain. Methods Immunohistochemistry and immunofluorescence were used to observe the distribution of AQP5 in brain of adult Wistar rat. Western blotting was applied to detect the expression of AQP5. At the same time, the distribution of AQP4 was observed as a control. Results AQP5 was distributed in cerebral pia mater, choroid plexes， perivascular tissuse, the pyramidal cell layer of hippocampus, granular cell layer and polymorphic cell layer of dentate gyrus, supraoptic nucleus, suprachiasmatic nucleus, bilateral cortexes alongside cerebral longitudinal fissure,which is similar to AQP4. In addition, AQP5 was lonely expressed in the nerve cell under cerebral cortex. The western blotting showed that expression of AQP5 was weaker than that of AQP4 in brain. Conclusions AQP5 was widely distributed in brain, as a coordinator of AQP4, which may be involved in water transport and balance, the produce and obsorption of CSF and the regulation of osmotic pressure.