1D小鼠发病过程中CD4记忆性T细胞的作用

Objective: Objective: In this paper, we aimed to study the function of CD4 memory T cells in the progress of T1D mice. Methods: The mice were confirmed T1D by blood glucose checked by blood sugar machine. The blood, spleens and pancrease were prepared for CD4 Tm test by immunostaining and FACS; The CD4 Tm from spleens were seperated and purified by CD4 Tm isolation kit and proliferation assay after CFSE staining was performed; The pancrease section were made for infiltrated lymphocytes testing. Results: Following the process of T1D, the percentage of CD4 Tm cells in blood increased significantly; There was no differences for the percentage of CD4 Tm cells in spleens; the percentage of CD4 Tm cells in pancrease increased significantly. The purified CD4 Tm cells from spleens proliferated with the stimulation of insulin peptide, but no differences with the stimulation of anti-CD3 mAb. Following the process of T1D, the infiltrated lymphocytes in pancrease increased in the pancrease sections.