斜纹夜蛾核型多角体病毒Ⅱ型（SpltNPVⅡ）orf29基因的研究

Spodoptera litura nucleopolyhedrovirusⅡ（SpltNPVⅡ）which has 146 ORFs was a new kind of baculovirus with host specificity and strong pest-killing abilities. And orf29 was a unique and new unknown gene which encoded 211 Aa and had never been reported. Bioinformations suggested ORF29 belonged to DU1251 superfamily, didn't have transmembrane domain and signal peptide and had the highest similarity with SeMNPV-ORF29. In this paper, Luciferase reporter gene detecting system was used to analyse promoter activity of the orf29's leader sequence 29P. The result showed 29P was a late promoter and the orf29 was assumed as a late gene. orf29's transcription phase was determined by realtime-PCR. Western blot was used to detected expression phase of orf29. It was confirmed that the transcription and expression of gene orf29 had started from 18 hours later after the virus infected the spli cells until the cells died. Finally, we carried out cellular localization about orf29 in spli cell, and the result of the immunocytochemistry suggested that GP29 exsited in cytoplasm more than in cell nucleus.