神经生长因子及其受体在蛋鸡生殖器官上的表达
Expression of Nerve Growth Factor (NGF), and Its Receptors TrkA and p75 in the Reproductive Organs of Laying Hens
为了研究神经生长因子（NGF）及其受体在蛋鸡等级前卵泡和输卵管上的表达，本试验以5只130日龄产蛋为试验动物，通过免疫组织化学方法和荧光定量PCR技术研究NGF及其受体酪氨酸激酶（TrkA）和神经营养素受体p75（p75）在蛋鸡等级前卵泡和输卵管上的表达定位及其mRNA表达水平。试验结果显示：NGF及其受体在各等级前卵泡的颗粒细胞和膜细胞以及输卵管各部分的上皮细胞中均有表达，输卵管上皮细胞中的表达量较弱。在各等级前卵泡和输卵管的不同部位NGF及其受体 mRNA表达量有差异，在大白卵泡中，NGF和p75的表达量较高（P < 0.05），而在小黄卵泡中TrkA的表达量最高（P < 0.05）。在输卵管中，NGF在漏斗部表达量最高而在峡部表达量最低（P < 0.01）。TrkA在膨大部表达量最高而在漏斗部表达量最低P < 0.01）。综上所述，NGF及其受体在蛋鸡生殖器官上均有不同程度的表达，由此推测NGF及其受体在蛋鸡生殖调节中可能有一定的作用。
In order to investigate the expression levels of nerve growth factor (NGF) and its receptors (TrkA and p75) in the prehierarchical follicle and oviducts of hens, five 130-day-old laying hens were examined by immunohistochemistry and RT-PCR analyses. NGF and its receptors were expressed in the theca cells and granulosa cells of prehierarchical follicles, and they also expressed in the epithelial cells of oviducts. The expressions of NGF mRNA were discrepant in different parts of the prehierarchical follicle and oviducts. The expression of NGF and p75 mRNA levels in large white follicle (LWF) were high, as well as TrkA in small yellow follicle (SYF). In oviduct, the expression of NGF is highest in infundibulum but lowest in magnum. These results suggest that NGF may play an important role in the regulation of reproduction in hens.
瞿长伟、李春梅、普少瑕
畜牧、动物医学生理学生物科学研究方法、生物科学研究技术
繁殖神经生长因子rkAp75等级前卵泡输卵管
henNGFoviductprehierarchical folliclesp75TrkA
瞿长伟,李春梅,普少瑕.神经生长因子及其受体在蛋鸡生殖器官上的表达[EB/OL].(2014-07-17)[2025-08-22].http://www.paper.edu.cn/releasepaper/content/201407-228.点此复制
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