牛肝中阿维菌素类兽药残留检测的改良高效液相色谱法研究
etection of Avermectins Ｒesidues in Beef Liver by Using Improved HPLC
目的：对牛肝中阿维菌素类兽药残留检测的国家标准高效液相色谱法进行改良，建立一种操作方便、耗时短且高灵敏度的牛肝中阿维菌素类药物残留检测的新方法。方法：样品经两次乙腈提取后，用乙腈-水-三乙胺储存备用，用Ｃ18SPE柱分离，1-甲基咪唑-乙腈、三氟乙酸酐-乙腈、冰乙酸和三乙胺衍生化，用乙腈作流动相，进行高效液相色谱法检测。结果：本方法中，爱普菌素、阿维菌素、多拉菌素、伊维菌素的标准曲线在2~200ng/ml的范围内，呈线性相关，相关系数均大于0.9992；4种药物的最低检测限均为2μg/kg；平均添加回收率在83~93%，批内变异系数均小于14％，批间变异系数均小于11%。结论：此改良法可作为牛肝中阿维菌素类兽药残留检测的参考方法。
Objective: In order to develop a simple, rapid and exact method for detecting the residues of avermectins in beef liver, the national standard method of HPLC was improved. Methods: After twice extracts by acetonitrile, the samples were storaged by acetonitrile-water- trimethylamine. And then separated by C18cartridge column. The amount of avermectins were detected by HPLC with acetonitrile being mobile phase after derived by 1-Methylimidazole-acetonitrile, trifluoroacetic anhydride- acetonitrile, glacial acetic acid-acetonitrile. Results: The standard carve of eprinomectin, avermectin, doramectin and ivermectin showed linear carve between 2 ng/mL and 200 ng/mL. The correlation coefficients were greater than 0.9992. The detection limits of these 4 drugs were 2μg/kg. The recoveries determined were between 83% and 93%, and the coefficient of variations of intra and inter were less than 14% and 11%. Conclusion: It can be a referential method to detect the residues of avermectins in beef liver.
谭梅、岳秀英、李燕君、吕晓华、吴晓岚、李然
畜牧、动物医学药学
牛肝阿维菌素类高效液相色谱法改良
Beef liverAvermectinsHPLCImprovement
谭梅,岳秀英,李燕君,吕晓华,吴晓岚,李然.牛肝中阿维菌素类兽药残留检测的改良高效液相色谱法研究[EB/OL].(2018-12-14)[2025-08-02].http://www.paper.edu.cn/releasepaper/content/201812-45.点此复制
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